lsolation of CA 125 from Breast Tumor Tissue Using GelChromatographl'

Abstract

Gel filtration chromatography technique was trsed (Sepharose CL - 68) of CA 125
from malignant breast tLrmor homogenate and the isolation of (CA 125 I r25l - anti CA 125
antibody), complex was prepared from crude CA 125 , isolated CA 125 and standard human CA
125. The results revealed the presence of two forms of CA 125 (AgI & AgII) and found to be 517
Kda and 38 Kda respectively: The isolation of CA 125 shorved a purification fold of 17.5 AgI and
15 for AgII.